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Orderable Name VZIKS Zika Virus, PCR, Molecular Detection, Serum


Ordering Guidance


Due to similar clinical presentations, testing for RNA or IgM-class antibodies to dengue and chikungunya viruses, concurrently with Zika virus testing, is strongly recommended.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Qualitative detection of Zika virus RNA in serum from individuals meeting the Centers of Disease Control and Prevention Zika virus clinical or epidemiologic criteria

Testing Algorithm

The following algorithms are available:

-Assessment for Zika Virus Infection

-Mosquito-borne Disease Laboratory Testing

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)/DNA Probe Hybridization

Specimen Type

Serum

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

 

Reference values apply to all ages.

Day(s) Performed

Tuesday, Thursday

Report Available

Same day/1 to 5 days

CPT Code Information

87662

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.