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Orderable Name TRECS T-Cell Receptor Excision Circles Analysis, Blood


Additional Testing Requirements


This assay is useful for evaluating thymic output, and for longitudinal assessment of thymic function.

 

For comprehensive assessment of thymic function in pediatric patients and/or individuals who have received hematopoietic stem cell transplantation, order this test together with CD4RT / CD4 T-Cell Recent Thymic Emigrants, Blood.



Shipping Instructions


Specimens must be received in the laboratory on weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of collection.

 

Samples arriving over the weekend or on observed holidays may be canceled.



Necessary Information


Ordering physician's name and phone number are required.

 

TREC Assay Patient Information (T589) is required. Testing will proceed without the form; however, results will be held under the information is received.



Specimen Required


For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Container/Tube: Lavender top (EDTA)

Specimen Volume:

Adults: 10 mL

Pediatrics

-Preferred volume for >1 year: 5 mL

-Preferred volume for ≤1 year old: 3 mL

Collection Instructions:

1. Do not collect specimen using a butterfly needle.

2. Send whole blood specimen in original tube. Do not aliquot.


Forms

TREC Assay Patient Information (T589) is required

Useful For

Measuring T-cell output or reconstitution (thymopoiesis) following hematopoietic cell transplantation or highly active antiretroviral therapy

 

Evaluating thymic function in patients with cellular or combined inborn errors of immunity (formerly primary immunodeficiencies), or receiving immunotherapy or cancer vaccines

 

Assessing T-cell recovery following thymus transplants for DiGeorge syndrome

Special Instructions

Method Name

Real-Time Quantitative Polymerase Chain Reaction (PCR)

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

Adults: 10 mL
Pediatrics: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 48 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) Performed

Varies

Report Available

6 to 8 days

CPT Code Information

81479-Unlisted molecular pathology procedure