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HelpOrderable Name NAIFA Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum
Additional Codes
CNE Order Code: ANA HEp-2
Useful For
Evaluation of patients suspected of having systemic autoimmune rheumatic disease (ANA-associated rheumatic diseases or connective tissue disease) or organ-specific autoimmune diseases such as autoimmune liver diseases
Method Name
Indirect Immunofluorescence
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
<1:80 (Negative)
Day(s) Performed
Monday through Saturday
Report Available
2 to 3 daysCPT Code Information
86039
Forms
If not ordering electronically, complete, print, and send General Test Request (T239) with the specimen.
Testing Algorithm
For more information see Connective Tissue Disease Cascade.