-1643205915.png){kind=logo}
Home
HelpOrderable Name MPLVS MPL Exon 10 Mutation Detection, Varies
Additional Codes
CNE Order Code: MPL,Exon10
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Peripheral blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 3 mL
Collections Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collections Instructions:
1. Invert several times to mix bone marrow.
2. Send specimens in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2- mL tube
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA from blood or bone marrow and provide indication of volume and concentration of DNA.
Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Aiding in the distinction between a reactive cytosis and a myeloproliferative neoplasm
Testing Algorithm
For more information see:
-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
Special Instructions
Method Name
Sanger Sequencing
Specimen Type
VariesSpecimen Minimum Volume
Blood, Bone marrow: 0.5 mL; Extracted DNA: 50 mcL at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Bone marrow biopsies Slides paraffin shavings Moderately to severely clotted |
Reject |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
5 to 8 daysCPT Code Information
81339-MPL (myeloproliferative leukemia virus oncogene, thrombopoietin receptor, TPOR) (eg, myeloproliferative disorder), exon 10 sequence