Notes

Modified Two-Tiered testing algorithm (MTTTA) starts with an initial screen for the detections of total antibodies against the Borrelia Vise/pepC10 proteins. Samples that are screen positive or equivical by this first tier EIA are subsequently reflexed to 2 separate EIAs for IgM and IgG antibodies against B. burgdorferi whole sell sonicate material. Western blot is no longer recommended.