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Orderable Name HRTVS Heartland Virus, RNA, Molecular Detection, PCR, Serum


Ordering Guidance


Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time polymerase chain reaction) and may require serologic testing, which is available through the Centers for Disease Control and Prevention.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection centrifuge and aliquot serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Aiding in the diagnosis of central nervous system infection caused by Heartland virus using serum specimens

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Specimen Type

Serum

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 5 days

CPT Code Information

87798

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.