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Orderable Name HIQNP HIV-1 RNA Detection and Quantification, Prenatal, Plasma


Ordering Guidance


For detection and quantification of HIV-1 RNA in plasma before and during antiretroviral therapy in pregnant individuals only. For nonpregnant individuals, order HIVQN / HIV-1 RNA Detection and Quantification, Plasma.



Shipping Instructions


Ship specimen frozen on dry ice. If shipment will be delayed for more than 24 hours, freeze plasma specimen at -20 to -80° C until shipment



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Freeze aliquoted plasma for shipment.


Useful For

Diagnosis of HIV-1 infection in pregnant individuals with acute or early HIV-1 infection

 

Quantifying plasma HIV-1 RNA levels (viral load) in pregnant individuals living with HIV-1:

-Before initiating anti-HIV-1 drug therapy (baseline viral load)

-Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy

-Who may be noncompliant with anti-HIV-1 drug therapy

 

Monitoring HIV-1 disease progression before or during antiretroviral drug therapy in pregnant individuals

Method Name

Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 84 days
  Refrigerated  6 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Undetected

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

CPT Code Information

87536

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.