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Orderable Name HDVQU Hepatitis D Virus RNA Detection and Quantification, Real-Time RT-PCR, Serum


Ordering Guidance


This test should be requested for all individuals who are newly confirmed to be positive for hepatitis B virus surface antigen (HBsAg) or HDV total antibodies in serum.



Shipping Instructions


1. Freeze serum immediately, and ship specimen frozen on dry ice.

2. If shipment will be delayed for more 24 hours, freeze serum at -20 to -80° C (up to 35 days) until shipment on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 2.2 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.


Useful For

Detection of hepatitis D virus (HDV) for diagnosis of acute or chronic hepatitis D

 

Determine HDV RNA level in serum to monitor progression and response to antiviral therapy for chronic hepatitis D

Testing Algorithm

For more information see Hepatitis D: Diagnostic Testing Algorithm.

Method Name

Real-Time, Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Specimen Type

Serum SST

Specimen Minimum Volume

1.0 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred) 35 days ALIQUOT TUBE
  Refrigerated  5 days ALIQUOT TUBE

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Reference Values

Undetected

Day(s) Performed

Varies (once per week)

Report Available

1 to 10 days

CPT Code Information

87523