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HelpOrderable Name EGFRW EGFR Targeted Mutation Analysis with ALK Reflex, Tumor
Necessary Information
A pathology report must accompany specimen for testing to be performed.
Specimen Required
Preferred: Submit both of the following specimens.
Acceptable: Submit at least one of the following specimens.
Specimen Type: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue.
Specimen Type: Tissue slide
Slides: 1 Hematoxylin and eosin-stained and 10 unstained
Collection Instructions:
Collection Instructions:
Submit the followings slides:
1 Slide stained with hematoxylin and eosin
AND
10 Unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.
Note: The total amount of required tumor nuclei can be obtained by scraping up to 10 slides from the same block.
Additional Information: Unused unstained slides will not be returned.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Molecular Genetics: Inherited Cancer Syndromes Patient Information (T519)
Oncology Test Request (T729)
Useful For
Identifying non-small cell lung cancers that may benefit from treatment with epidermal growth factor receptor -targeted therapies or anaplastic lymphoma kinase inhibitors
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SLIRV | Slide Review in MG | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| LCAF | ALK (2p23), Lung Cancer, FISH, Ts | Yes | No |
Testing Algorithm
When this test is ordered, the EGFR Gene, Mutation Analysis, 51 Mutation Panel, Tumor will always be performed. All specimens without an EGFR mutation will be automatically reflexed to testing for the ALK (2p23) rearrangement. Specimens with an identified EGFR mutation will result in cancellation of the reflex test.
When this test is ordered, a slide review will always be performed at an additional charge.
Method Name
Polymerase Chain Reaction (PCR)
Specimen Type
VariesSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
5 to 14 daysCPT Code Information
81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants(eg, exon 19 deletions, L858R, T790M, G719S, L861Q)
88381-Microdissection, manual
88271 x 2-DNA Probe (if appropriate)
88274-Interphase in situ hybridization (if appropriate)
88291-Interpretation and report (if appropriate)