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Orderable Name EEEV1 Red Blood Cell (RBC) Enzyme Evaluation, Blood


Necessary Information


Metabolic Hematology Patient Information (T810) is strongly recommended and should include clinical history. Testing may proceed without this information, however if the information requested is received, it allows for a more complete interpretation.



Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: Yellow top (ACD solution A)

Specimen Volume: 12 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Forms

1. Metabolic Hematology Patient Information (T810)

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.

Useful For

Identifying defects of red blood cell enzyme metabolism

 

Evaluating patients with Coombs-negative hemolytic anemia

Profile Information

Test ID Reporting Name Available Separately Always Performed
EEEVI Erythrocyte Enzyme Interpretation No Yes
G6PDC G6PD Enzyme Activity, B Yes, (Order G6PD1) Yes
PKC PK Enzyme Activity, B Yes, (Order PK1) Yes
GPIC Glucose Phosphate Isomerase, B Yes, (Order GPI1) Yes
HKC Hexokinase, B Yes, (Order HK1) Yes
AKC Adenylate Kinase, B Yes, (Order AK1) Yes
PFKC Phosphofructokinase, B Yes, (Order PFK1) Yes
PGKC Phosphoglycerate Kinase, B Yes, (Order PGK1) Yes
TPIC Triosephosphate Isomerase, B Yes, (Order TPI1) Yes
GSH Glutathione, B Yes Yes
P5NT Pyrimidine 5' Nucleotidase, B Yes Yes

Testing Algorithm

This is a consultative evaluation in which the case will be evaluated at Mayo Clinic Laboratories.

Method Name

EEEVI: Medical Interpretation

G6PDC, GPIC, PKC, HKC, AKC, PFKC, PGKC, TPIC, GSH, P5NT: Kinetic Spectrophotometry

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 11 days

Reject Due To

Gross hemolysis Reject

Reference Values

Definitive results and an interpretive report will be provided.

CPT Code Information

82955-G6PD Enzyme Activity

84087-Glucose phosphate isomerase

84220-Pyruvate Kinase Enzyme Activity

82657-Hexokinase

82657-Adenylate Kinase

82657-Phosphofructokinase

82657-Phosphoglycerate Kinase

82657-Triosephosphate Isomerase

82978-Glutathione

83915-Pyrimidine 5' Nucleotidase

Day(s) Performed

Tuesday, Thursday

Report Available

2 to 13 days