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Orderable Name DMTBR Mycobacterium tuberculosis Complex, Molecular Detection of Drug Resistance Markers, Next-Generation Sequencing


Ordering Guidance


This test is used to identify the drug resistance variants in an M tuberculosis complex culture isolate.



Shipping Instructions


1. For shipping information see Infectious Specimen Shipping Guidelines

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and organism identification are required.



Specimen Required


Supplies: Infectious Container, Large (T146)

Specimen Type: Mycobacterium tuberculosis complex isolate growing in pure culture

Container/Tube:

Preferred: Solid slant medium (eg. Middlebrook 7H10, 7H11 or Lowenstein Jensen agar)

Acceptable: Broth medium (eg. Mycobacteria Growth Indicator Tube, 7H9 broth, BACT/ALERT MP or VersaTREK bottle). Note: Broth specimens will require subculture which will delay results.

Specimen Volume:

Solid slant medium: Isolate with visible growth

Broth medium: Greater than or equal to 3 mL of broth culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.

3. Turnaround time for results may be delayed if subculture is needed to ensure purity.


Useful For

Molecular detection of drug resistance variants in culture isolates of the Mycobacterium tuberculosis complex

 

May provide a more rapid detection of drug resistance than phenotypic, broth-based testing for selected gene targets

 

Aiding in the resolution of discrepant results obtained using phenotypic methods

 

Testing for M tuberculosis complex isolates that are not sufficiently viable to allow for culture-based testing

Testing Algorithm

Next-Generation sequencing (NGS) of Mycobacterium tuberculosis complex isolates is performed followed by evaluation of selected genes of interest for the presence of well-characterized, drug resistance-conferring variants.

Method Name

Next-Generation Sequencing (NGS)

Specimen Type

Varies

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject
Mixed culture Reject

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

Report Available

7 to 14 days

CPT Code Information

87153 - M tuberculosis drug resistance, NGS